New Nickel column packing New 6xHis-tag purification media
Release time: 2016-08-24
In the past, we used to purify proteins with nickel columns, and after each purification, we had to remove the nickel before we could clean the columns with sodium hydroxide and then chelate on the nickel ions. Its process is tedious and time consuming. The reason why it takes so much effort is that nickel ions are reduced when they encounter sodium hydroxide and the column turns black. Then whether our nickel column is also able to be cleaned directly with NaOH online as well as ion exchange? The answer is yes, Wuhan Jingcheng Biotechnology Co., Ltd. has newly developed a nickel column purification packing, the packing combination principle is the same as the common NTA packing, the only difference is that we can achieve online CIP without nickel removal, and keep the load does not drop.
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6xHis Labeled Protein Nickel Column Purification Method Protocol
1. Solution preparation: 1.1 Binding Buffer: 10mM PB, 500mM NaCl, 10mM imidazole, pH7.4. 1.2 Elution Buffer1: 10mM PB, 500mM NaCl, 50mM imidazole, pH 7.4.
Jingcheng CrystaroseC FF filler test report
1、Apparatus and consumables 1.1 Instrument: JC-1 automated protein purification system (Jingcheng) 1.2 Chromatographic column: XK16 (GE) 1.3 Jingcheng Biotechnology: CrystaroseC FF(5ml)
Metal chelate chromatography affinity packing comparable to imported products
CrystaroseC FF metal chelate chromatography affinity packing has super high rigidity, higher linear flow rate up to 700cm/h, and the back pressure is close to the imported similar products or even lower. Therefore, the packing life is longer.
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E-mail：122460871@qq.com
Address: Building B, R&D Area, Wuhan National Biological Industry Base Project, No. 666 Gaoxin Avenue, East Lake High-tech Development Zone, Wuhan City
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