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SP Crystarose HP（Strong Cation Exchange Resin）

classification：Agarose packing

SP Crystarose Fast Flow is a strong cation exchange chromatographic packing

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Product Overview

SP Crystarose HP is a strong cation exchange chromatography resin composed of porous agarose with an average particle size of approximately 35 µm, chemically bonded with weak cationic functional groups. The molecular formula of the functional groups is: -O-CH₂CHOHCH₂OCH₂CH₂CH₂SO₃-.

Compared to imported fillers of the same type, it offers a higher specific surface area and better biocompatibility. It maintains a higher loading capacity at high flow rates while also achieving high resolution. Due to its large specific surface area, equilibration and elution times are also shorter.

Product features: high flow rate, high rigidity, high loading capacity, excellent physical and chemical stability, long service life, good reproducibility; easy to scale up.

Product Components

Components	C119001	C119002	C119003
SP Crystarose HP	100ml	500ml	1L

Storage conditions

The product can be stored long-term at 4°C in 20% ethanol.

Scope of Application

SP Crystarose HP series media is a highly cross-linked agarose-based matrix with high flow rates and low back pressure. It can be CIP cleaned under 1M sodium hydroxide conditions and has excellent physical and chemical properties and rheological properties. It is commonly used for sample capture and intermediate purification and is a classic ion exchange filler widely used in preparative protein separation research and industrial applications.

Technical specifications

Specification	Details
Matrix	Highly cross-linked 6% agarose
Average Particle Size	35 µm
Ligand Density	0.16-0.23 µmol/mL resin
Dynamic Binding Capacity	55 mg/mL ribonuclease
Compatible Buffers	All standard buffer systems
Chemical Stability	Stable in: all common buffers; 1M NaOH; 0.2M HCl; 2% SDS; 10 mM β-mercaptoethanol; 40% isopropanol; 70% ethanol; 8M urea; 6M guanidine HCl (1 week at 40°C)
pH Stability (CIP)	4-13 (3-14)
Maximum Pressure	0.35 MPa
Maximum/Recommended Flow Rate	200 cm/h (max); 150 cm/h (recommended)
Storage Solution	20% ethanol
Storage Temperature	0-30°C

Key words：

SP-Crystarose-High-performance