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Q Agarose gel HP（Strong Anion Exchange Resin）

classification：Agarose packing

Q- Crystarose HP is a strong anion exchange chromatography packing, it uses porous agarose with an average particle size of 90um formed by chemically bonding anionic and cationic functional ligands

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Q-Crystarose High performance

Q Agarose Gel HP

I. Introduction

Q- Crystarose HP is a strong anion exchange chromatography packing, it uses porous agarose with an average particle size of 90um formed by chemically bonding anionic and cationic functional ligands, the group is: -O-CH2CHOHCH2N+(CH3)3, which has higher specific surface area and better biocompatibility, it then maintains a higher loading capacity at high flow rates and at the same time has a high resolution. Due to the large specific surface area, the equilibration and elution time is also shorter.

Features of this product: fast flow rate, high rigidity, high loading capacity, good physical and chemical stability, long life, good repeatability; easy to amplify.

II. Product technical specifications:

Test environment: column inner diameter 5cm, height 15cm, pressure 1bar

Third, the application of precautions:

1) Column filling

1, the column should be filled with a small amount of buffer solution at the lower end of the column before filling, discharging the air bubbles inside the column, closing the column outlet, retaining a small amount of buffer solution in the column. You can add a little Tween inside to avoid bubble generation, or you can switch to pure water to load the column. Before loading the column can be pumping funnel will be gum with buffer solution or pure water pumping wash 3-5 times the volume of gum.

2、Pour the packing into the chromatography column, if necessary, a glass rod can be used close to the inner wall of the column to divert the flow, in order to reduce the generation of bubbles, you can let the packing settle naturally, but also the process of loading the column can apply a certain pressure to accelerate the speed of loading the column, which can improve a certain column efficiency. Note that the maximum flow rate of the chromatography in the chromatography process should be less than the flow rate when loading the column.

3. Before loading the column, the packing material should be placed at room temperature for 1-3 hours after being taken out of the refrigerator to keep the packing temperature consistent with the ambient temperature, which can avoid a large number of air bubbles due to the drastic temperature change when loading the column, thus affecting the column efficiency.

2) Protein binding

The salt concentration and pH of the sample should be consistent with the buffer of the equilibrium column as much as possible, too high salt concentration or too low pH may affect the binding effect of the column, so you should make appropriate adjustments according to your sample.

3) Protein elution

1, ion exchange is generally low salt adsorption high salt elution. If the linear gradient elution is used, the upper sample volume should be controlled at 10% of the total loading of the column packing or lower, the upper sample volume is low, which can effectively improve the separation effect, and the sample concentration should not be too high. If the stage elution method is used, the upper sample volume can be controlled at about 30% of the total column packing capacity. Stage elution is relatively easier to scale up and reproducible. The loading height of ion exchange chromatography columns generally does not exceed 30 cm, and to increase the column size only the column diameter can be increased. Linear gradient elution and phase elution methods have their advantages and disadvantages, generally linear gradient elution method is suitable for figuring out the elution conditions, while phase elution is suitable for large-scale purification.

Fourth, regeneration, cleaning

After the chromatographic column is used up, it is washed with 0.2-0.5M NaOH (1M NaCl can be added), DH?2O and 0.1-0.2M HCL for 3-5 bed volumes, then 1M NaCL is equilibrated to pH neutral and 3 bed volumes are rinsed with pure water and 20% each and stored. Procedure: Wash 3-5 column volumes with NaOH, then wash 3-5 column bed volumes with water, then wash 3-5 column volumes with HCL. If you encounter stubborn substances that cannot be eluted by the above method, you can add an appropriate decontaminant to the cleaning solution, and the liquid can be used directly to clean the column with 6M guanidine hydrochloride or 8M urea. Finally 20% ethanol flow wash 5 column bed volume and save.

V. Storage

Store in 20% ethanol at 4°C for long term storage.

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Q-Crystarose High performance