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DS-10 Desalting Column

classification：Gel filtration chromatography pre-assembled columns

DS-10 desalination column is a convenient manual desalination column, all operations can be completed without borrowing instruments, high desalting efficiency, convenient and fast.

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Product Overview

The DS-10 Desalting Column is a manual, instrument-free solution for rapid and efficient desalting and buffer exchange. Prepacked with 8.5 mL of Crystadex G-25 resin (exclusion limit: 5,000 Da), it separates molecules by size:Small molecules (<5,000 Da) enter the resin pores, following a longer path (total gel volume).Large molecules (>5,000 Da) bypass pores and elute faster (outer volume).

Product Components

Product Name	Catalog No.	Packaging	Loading Volume	Molecular Weight Range	Particle Size
DS10 G-25	D010005	6 columns	≤3ml	>5000Da	40-160
DS10-G-25	D010006	30 columns	≤3ml	>5000Da	40-160
DS10 G-50	D010012	6 columns	≤3ml	>30000Da	60-250
DS10 G-50	D010013	30 columns	≤3ml	>30000Da	60-250

Operation Steps

Equilibration:Remove the upper and lower caps. The protective solution in the column will drain naturally.Continue adding buffer solution while monitoring the flow until the UV absorption baseline stabilizes, or until 3 column volumes have passed. This step removes residual protective solution.
Sample Loading:The maximum loading volume for DS 10 is 2.5 mL. If the sample volume is less than 2.5 mL, add buffer to reach 2.5 mL after the sample has fully entered the resin bed.
Elution:Continue adding buffer for elution. Refer to Figure 1 for the elution curve.Note 1: The "buffer" refers to the desired solution (e.g., deionized water if desalting into pure water).Note 2: The general rule is to collect eluate at ~120% of the sample volume. For example, for a 2 mL sample, start collecting at 2.8 mL and stop at 5.2 mL.
Regeneration & Storage:

Although designed for single use, the column can be regenerated:

Wash with 5 column volumes (CV) of deionized water.
Clean with 2–3 CV of 0.2 M NaOH.
Rinse with 3 CV of deionized water.
Store with 5 CV of 20% ethanol.

Figure 1

Note: For a 2.5 mL sample, after full loading, add 3.5 mL of buffer for elution. By excluding peak leading/tailing regions, protein concentration can be increased while reducing elution volume.

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