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Crystaldex G25

classification：Dextran gel packing

G25 dextran gel does not require swelling and can be used directly in columns. It has high desalting efficiency, high protein recovery rate, and is inexpensive, making it suitable for small-scale use in laboratories.

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Product Overview

Gel filtration chromatography (also known as molecular sieving) works on the principle that small molecules can pass through the pores of a porous gel, while large molecules cannot. Additionally, the time it takes for molecules to pass through the gel pores varies depending on their molecular weight and geometric shape, thereby separating samples of different molecular weights and structures. Crystaldex G25 utilizes gel filtration to remove salts, labels, and other small molecules (MW < 5000 Da) from nucleic acids, proteins, and other large molecules, or for buffer exchange.

The finer the dextran G25 resin particles, the higher the resolution and separation efficiency, making it more suitable for separation experiments involving molecules with similar molecular weights; desalting and buffer exchange typically use medium-sized or coarse-sized resin particles.

Product Components

Product Name	Catalog No.	Packaging	Particle Size
Crystaldex G25 (Superfine)	C211001	100g	20-75µm
	C211002	500g
	C211003	5kg
	C211004	100ml
	C211005	500ml
Crystaldex G25 (Fine)	C214001	100g	35-140µm
	C214002	500g
	C214003	5kg
	C214004	100ml
	C214005	500ml
Crystaldex G25 (Medium)	C213001	100g	90-250µm
	C213002	500g
	C213003	5kg
	C213004	100ml
	C213005	500ml
Crystaldex G25 (Coarse)	C212001	100g	170-250µm
	C212002	500g
	C212003	5kg
	C212004	100ml
	C212005	500ml

Storage Conditions

The liquid is stored long-term in 20% ethanol at 0-30°C; the dry powder is stored long-term at 0-30°C.

Technical Specifications

Specification	Details
Matrix	Cross-linked dextran
Average Particle Size	75 µm
Particle Size Range (wet)	15-70 µm (Superfine) 40-160 µm (Fine) 90-260 µm (Medium) 170-520 µm (Coarse)
Separation Range (globular proteins)	1,000-5,000 Da
Separation Range (dextrans)	100-5,000 Da
Compatible Buffers	All standard buffer systems
Chemical Stability	Stable in: • All common buffers • 0.2 M NaOH • 0.2 M HCl
pH Stability (long-term)	2-13
CIP Stability (short-term)	2-13
Storage Conditions	0.01 M NaOH, 0-30°C

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