PRODUCTS

Ni Crystarose HP

classification：6xHis tag protein purification packing

classification：Affinity chromatography

特异性强，非特异性吸附低，用于纯化细胞培养液、细菌裂解液中的6*His标签蛋白，用于大规模纯化

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Product Overview

Immobilized Metal Affinity Chromatography (IMAC), also known as Fixed Metal Ion Affinity Chromatography (FMIC), separates proteins by leveraging the specific interaction between surface-exposed amino acids (primarily histidine) and transition metal ions (e.g., Ni²⁺, Co²⁺). Ni Crystarose HP is a highly cross-linked agarose matrix pre-charged with Ni²⁺ ions and functionalized with metal-chelating groups for efficient His-tagged protein purification.

Product Components

Component	C521001	C521002	C521003
Ni Crystarose HP	10 ml	50 ml	500 ml

Product Features

a. High rigidity, fast flow rate, and high loading capacity.

b. Extremely low nickel ion leakage; typically, no re-chelating of nickel ions is required during normal service life.

c. Lower non-specific adsorption—in most cases, the wash step can be omitted, and the service life is longer.

d. Higher specificity, superior purity, increased loading capacity, and the imidazole elution concentration is reduced by half compared to conventional resins.

e. Broad reagent compatibility, including compatibility with reducing agents and EDTA.

f. Direct cleaning-in-place (CIP) with 0.5M NaOH without the need for nickel stripping—fewer steps, reduced contamination, and easier scale-up production. This eliminates the drawback of other nickel affinity resins (immobilized metal affinity chromatography resins) that require nickel removal before cleaning, thereby extending service life and improving loading capacity.

g. Enhanced binding specificity, compatible with complex samples. Capable of capturing His-tagged proteins even in the presence of up to 5 mM EDTA-Na₂, delivering unmatched purification performance even in highly challenging cell culture lysates.

Storage Conditions

Store long-term in 20% ethanol at 4°C.

Applications

The Ni Crystarose series represents a next-generation resin for His-tagged protein purification, offering significant advancements over traditional methods

Technical Specifications

Matrix	Highly cross-linked 6% agarose
Particle Size	25–45 µm
Ligand Density	0.16–0.23 µmol/mL resin
Dynamic Binding Capacity	>40 mg His-tagged protein/mL resin
Compatible Buffers	All standard buffer systems
Chemical Stability	Stable in: 1M NaOH, 0.2M HCl 2% SDS, 10 mM β-mercaptoethanol 40% isopropanol, 70% ethanol 8M urea, 6M guanidine HCl (40°C for 1 week)
pH Stability (CIP)	3–12 (short-term: 2–14)
Max Pressure	0.35 MPa
Max Flow Rate	150 cm/h
Storage Solution/ Storage Temperature	20% ethanol/0–30°C

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