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Chelating Crystarose FF Resin

classification：6xHis tag protein purification packing

金属螯合亲和色谱，又称固定金属离子亲和色谱，其原理是利用蛋白质表面的一些氨基酸，如组氨酸能与多种过度金属离子Cu2+,Zn2+,Ni2+,Co2+发生特殊的相互作用，利用这个原理可以把富含这类氨基酸的蛋白质吸附，从而达到分离的目的。

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Product Overview

Metal chelation affinity chromatography, also known as fixed metal ion affinity chromatography, is based on the principle that certain amino acids on the surface of proteins, such as histidine, can form special interactions with various transition metal ions such as Cu²⁺, Zn²⁺, Ni²⁺, and Co²⁺. This principle can be used to adsorb proteins rich in these amino acids, thereby achieving the purpose of separation. For this reason, agarose gels conjugated with these metal ions can selectively separate proteins containing multiple amino acids, as well as peptides, proteins, and nucleotides that exhibit metal ion adsorption properties. Cysteine and tryptophan can also bind to immobilized metal ions, but this binding is significantly weaker than the binding strength between histidine residues and metal ions.

Chelating Crystarose resin is a resin without pre-chelated metal ions, allowing customers to chelate different metal ions as needed for protein purification. The resin’s features include: high loading capacity, fast flow rate, and high protein binding efficiency.

Characteristics:

1. Pre-chelated metal ion chromatography resin (Chelating), convenient to use;

2. Can be repeatedly chelated with multiple metal ions as needed;

3. Stronger protein binding, suitable for purifying His-tagged proteins with weaker binding;

4. When used with our dedicated sample diluent, adsorption efficiency is significantly improved;

5. High loading capacity and fast flow rate, with slight ion leakage.

6. Commonly used industrial-grade Ni column purification media, easy to use and cost-effective.

Product Components

Components	C541001	C541002	C541003
Chelating Crystarose FF	25ml	100ml	500ml

Storage conditions

The product can be stored long-term in 20% ethanol at 0-30°C.

Technical specifications

Specification	Details
Matrix	Highly cross-linked 4% agarose
Average Particle Size	90 µm
Particle Size Range	45-165 µm
Ligand Density	20 µmol Ni²⁺/mL resin
Dynamic Binding Capacity	40 mg/mL His-tagged protein
Compatible Buffers	All standard buffer systems
Chemical Stability	Stable in: all common buffers; 0.1M NaOH; 0.01M HCl (1 week at 40°C); 1M NaOH, 70% acetic acid (12 h); 2% SDS, 30% isopropanol (1 h)
pH Stability (long-term)	3-12
CIP Stability (short-term)	2-14
Maximum Pressure	0.4 MPa
Maximum/Recommended Flow Rate	750 cm/h (max); 150 cm/h (recommended)
Storage Solution	20% ethanol
Storage Temperature	0-30°C

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