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Ni Crystarose FF （His-Tag）

classification：6xHis tag protein purification packing

classification：Product Center

classification：Affinity chromatography

The principle of metal chelation affinity chromatography, also known as fixed metal ion affinity chromatography, is to use certain amino acids (mainly histidine) on the surface of proteins to have special interactions with a variety of excessive metal ions (such as Ni2), and use this principle to adsorb proteins rich in these amino acids to achieve the purpose of separation.

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Product Overview

Immobilized Metal Affinity Chromatography (IMAC), also known as Fixed Metal Ion Affinity Chromatography (FMIC), separates proteins by leveraging the specific interaction between surface-exposed amino acids (primarily histidine) and transition metal ions (e.g., Ni²⁺, Co²⁺). Ni Crystarose FF is a highly cross-linked agarose matrix pre-charged with Ni²⁺ ions and functionalized with metal-chelating groups for efficient His-tagged protein purification.

Product Components

Component	C520001	C520002	C520003
Ni Crystarose FF	10 ml	50 ml	500 ml

Product Features

a. High rigidity, fast flow rate, and high loading capacity.

b. Extremely low nickel ion leakage; typically, no re-chelating of nickel ions is required during normal service life.

c. Lower non-specific adsorption—in most cases, the wash step can be omitted, and the service life is longer.

d. Higher specificity, superior purity, increased loading capacity, and the imidazole elution concentration is reduced by half compared to conventional resins.

e. Broad reagent compatibility, including compatibility with reducing agents and EDTA.

f. Direct cleaning-in-place (CIP) with 0.5M NaOH without the need for nickel stripping—fewer steps, reduced contamination, and easier scale-up production. This eliminates the drawback of other nickel affinity resins (immobilized metal affinity chromatography resins) that require nickel removal before cleaning, thereby extending service life and improving loading capacity.

g. Enhanced binding specificity, compatible with complex samples. Capable of capturing His-tagged proteins even in the presence of up to 5 mM EDTA-Na₂, delivering unmatched purification performance even in highly challenging cell culture lysates.

Storage Conditions

Store long-term in 20% ethanol at 4°C.

Applications

The Ni Crystarose series represents a next-generation resin for His-tagged protein purification, offering significant advancements over traditional methods.

Technical Specifications

Specification	Details
Matrix	Highly cross-linked 6% agarose
Particle Size	45–165 µm
Ligand Density	15µmol Ni²⁺/ml
Dynamic Binding Capacity	40 mg His-tagged protein/mL resin
Compatible Buffers	All standard buffer systems
Chemical Stability	Stable in: 1M NaOH, 0.2M HCl 2% SDS, 10 mM β-mercaptoethanol 40% isopropanol, 70% ethanol 8M urea, 6M guanidine HCl (40°C for 1 week)
pH Stability (CIP)	3–12 (short-term: 2–14)
Max Pressure	0.4 MPa
Max Flow Rate	750cm/h
Storage Solution/ Storage Temperature	20% ethanol/0–30°C

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