PRODUCTS
CNBr-Activated Crystarose 4B
classification：Pre-activation chromatography
The active groups of the activated filler can bind to proteins, peptides, nucleic acids, and other ligands containing amino (NH2) groups.
Product Overview
CNBr-Activated Crystarose 4B is a CNBr-activated resin that uses agarose gel with an average particle size of 45–165 μm. The activated groups of this packing material can bind to proteins, peptides, nucleic acids, and other ligands containing amino (-NH₂) groups. Its advantages include mild reaction conditions, fast reaction rates, high activity, and high coupling efficiency. Unlike similar products on the market, it has extremely low ligand detachment after coupling.
Product Components
|
Product Name |
Catalog Number |
Package Size |
Application Characteristics |
|
CNBr-Activated Crystarose 4B |
C710001 |
5 g |
Coupling to -NH₂ groups |
|
C710002 |
10 g |
||
|
C710003 |
50 g |
||
|
C710004 |
100 g |
Scope of application
Self-made affinity chromatography fillers provide specific coupling solutions for different users.
Storage conditions
Store the product at 4°C. After coupling, it can be stored at 4°C in storage solution or 20% ethanol.
Technical specifications
|
Specification |
Details |
|
Matrix |
Agarose gel |
|
Applicable Range |
Substances with amino groups (polysaccharides, proteins, nucleic acids, antibodies, etc.) |
|
Coupling Group |
-NH₂ |
|
Coupling Capacity |
15-30 mg BSA/mL gel |
|
Average Particle Size |
90 µm |
|
Particle Size Range |
45-165 µm |
|
Maximum Flow Rate |
75 cm/h |
|
Coupling Conditions |
pH 8-10, >4°C for 16 h or >25°C for 1 h |
|
Resuspension Ratio |
1 g dry powder yields ~4-4.5 mL wet gel volume |
|
Storage Conditions |
4-8°C (dry, protected from light) for short-term; -20°C for long-term storage |
Key words：
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